hos human os cell lines  (ATCC)

Journal: Genes & Diseases

Article Title: Veratramine influences the proliferation of human osteosarcoma cells through modulating the PI3K/AKT signaling cascade

doi: 10.1016/j.gendis.2025.101630

Figure Lengend Snippet: Veratramine inhibits the proliferation of human osteosarcoma cells. (A – E) The effects of VER on the proliferation of 143B and HOS were detected by the crystal violet staining (A, B), colony formation assay (C, D), and CCK8 assay (E). (F, G) The effects of VER on the cell cycle of 143B and HOS were detected by flow cytometry assay. (H, I) The protein levels of proliferation-related molecules in 143B and HOS were tested by Western blot. (J) PCNA in the cytoplasm of 143B was detected by immunofluorescence test, with PCNA labeled with Cy-3 in red and the nucleus labeled in blue with DAPI. Results are presented as mean ± standard deviation. Statistical significance is indicated by ∗ p < 0.05 and ∗∗ p < 0.01 versus the control group.

Article Snippet: 143B and HOS human OS cell lines were purchased from the American Type Culture Collection (ATCC).

Techniques: Staining, Colony Assay, CCK-8 Assay, Flow Cytometry, Western Blot, Immunofluorescence, Labeling, Standard Deviation, Control

Journal: Genes & Diseases

Article Title: Veratramine influences the proliferation of human osteosarcoma cells through modulating the PI3K/AKT signaling cascade

doi: 10.1016/j.gendis.2025.101630

Figure Lengend Snippet: Veratramine suppresses the movement and penetration of human osteosarcoma cells. (A – D) The effects of VER on the migration abilities of 143B and HOS cells were detected by wound healing test. (E – H) The effects of VER on the migration and invasive abilities of 143B and HOS cells were detected by transwell assay. (I, J) The protein levels of matrix metallopeptidases (MMPs) and epithelial-mesenchymal transition-related molecules in 143B and HOS were tested by Western blot. (K) Vimentin and MMP-2 in the cytoplasm of 143B were detected by immunofluorescence test, with vimentin and MMP2 labeled with Cy-3 in red and the nucleus labeled in blue with DAPI. Results are presented as mean ± standard deviation. Statistical significance is indicated by ∗ p < 0.05 and ∗∗ p < 0.01 versus the control group.

Article Snippet: 143B and HOS human OS cell lines were purchased from the American Type Culture Collection (ATCC).

Techniques: Migration, Transwell Assay, Western Blot, Immunofluorescence, Labeling, Standard Deviation, Control

Journal: Genes & Diseases

Article Title: Veratramine influences the proliferation of human osteosarcoma cells through modulating the PI3K/AKT signaling cascade

doi: 10.1016/j.gendis.2025.101630

Figure Lengend Snippet: Veratramine promotes apoptosis of human osteosarcoma cells. (A – D) The effects of VER on the apoptosis of 143B and HOS cells were detected by Hoechst 33258 staining assay (A, B) and flow cytometry (C, D). (E) Representative transmission electron microscopy images of 143B cells in the control and VER group. (F, G) The protein levels of apoptosis-related molecules in 143B and HOS were tested by Western blot. (H) Detection of Bcl2 expression of 143B by immunofluorescence, labeled Bcl2 with Cy-3 (red) and labeled nucleus with DAPI (blue). Results are presented as mean ± standard deviation. Statistical significance is indicated by ∗ p < 0.05 and ∗∗ p < 0.01 versus the control group.

Article Snippet: 143B and HOS human OS cell lines were purchased from the American Type Culture Collection (ATCC).

Techniques: Staining, Flow Cytometry, Transmission Assay, Electron Microscopy, Control, Western Blot, Expressing, Immunofluorescence, Labeling, Standard Deviation

Journal: Genes & Diseases

Article Title: Veratramine influences the proliferation of human osteosarcoma cells through modulating the PI3K/AKT signaling cascade

doi: 10.1016/j.gendis.2025.101630

Figure Lengend Snippet: Molecular docking and PI3K/AKT signaling pathway validation. (A – G) Molecular docking of VER and AKT1, HSP90AA1, BCL2, EGFR, CCND1, JAK2, and PI3KCA. (H, I) The protein levels of PI3K/AKT-related molecules in 143B and HOS were tested by Western blot. (J) PI3K and AKT in the cytoplasm of 143B were detected by immunofluorescence test, with PI3K and AKT labeled in red with CY5 and the nucleus labeled in blue with DAPI. Results are presented as mean ± standard deviation. Statistical significance is indicated by ∗ p < 0.05 and ∗∗ p < 0.01 versus the control group.

Article Snippet: 143B and HOS human OS cell lines were purchased from the American Type Culture Collection (ATCC).

Techniques: Biomarker Discovery, Western Blot, Immunofluorescence, Labeling, Standard Deviation, Control